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Biosticker: patterned microfluidic stickers for rapid integration with microarrays
KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).ORCID-id: 0000-0002-0441-6893
Visa övriga samt affilieringar
2011 (Engelska)Ingår i: The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2011), Chemical and Biological Microsystems Society , 2011, s. 311-313Konferensbidrag, Publicerat paper (Refereegranskat)
Abstract [en]

We present a one-step, reversible, and biocompatible bonding method of a stiff patterned microfluidic "Biosticker", based on off-stoichiometry thiol-ene (OSTE) polymers [1], to state-of-the-art spotted microarray surfaces. The method aims at improving and simplifying the batch back-end processing of microarrays. We illustrate its ease of use in two applications: a high sensitivity flow-through protein assay; and a DNA-hybridization test. Read-out was performed in a standard highvolume array scanner, and showed excellent spot homogeneity and intensity. The Biosticker is aimed to be a plug-in for existing microarray platforms to enable faster protein assays and DNA hybridizations through mass transport optimization.

Ort, förlag, år, upplaga, sidor
Chemical and Biological Microsystems Society , 2011. s. 311-313
Nyckelord [en]
microarrays, lab-on-chip loc, OSTE, off-stoichiometry thiol-ene, microfluidic packaging
Nationell ämneskategori
Elektroteknik och elektronik
Identifikatorer
URN: urn:nbn:se:kth:diva-38609Scopus ID: 2-s2.0-84877953846ISBN: 9781618395955 (tryckt)OAI: oai:DiVA.org:kth-38609DiVA, id: diva2:437756
Konferens
The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2011)
Projekt
Positive
Anmärkning

QC 20110902

Tillgänglig från: 2011-08-30 Skapad: 2011-08-30 Senast uppdaterad: 2015-06-03Bibliografiskt granskad
Ingår i avhandling
1. Development of materials, surfaces and manufacturing methods for microfluidic applications
Öppna denna publikation i ny flik eller fönster >>Development of materials, surfaces and manufacturing methods for microfluidic applications
2011 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

This thesis presents technological advancements in microfluidics. The overall goals of the work are to develop new miniaturized tests for point-of-care diagnostics and robust super-lubricating surfaces for friction reduction. To achieve these goals, novel materials, surfaces and manufacturing methods in microfluidics have been developed.

Point-of-care diagnostic tests are portable miniaturized instruments that downscale and automate medical tests previously performed in the central laboratories of hospitals. The instruments are used in the doctor’s office, in the emergency room or at home as self-tests. By bringing the analysis closer to the patient, the likelihood of an accurate diagnosis, or a quick therapy adjustment is increased. Already today, there are point-of-care tests available on the market, for example blood glucose tests, rapid streptococcus tests and pregnancy tests. However, for more advanced diagnostic tests, such as DNA-tests or antibody analysis, integration of microfluidic functions for mass transport and sample preparation is required. The problem is that the polymer materials used in academic development are not always suited for prototyping microfluidic components for sensitive biosensors. Despite the enormous work that has gone into the field, very few technical solutions have been implemented commercially.

The first part of the work deals with the development of prototype point of-care tests. The research has focused on two major areas: developing new manufacturing methods to leverage the performance of existing materials and developing a novel polymer material platform, adapted for the extreme demands on surfaces and materials in miniaturized laboratories. The novel manufacturing methods allow complex 3D channel networks and the integration of materials with different surface properties. The novel material platform is based on a novel off-stoichiometry formulation of thiol-enes (OSTE) and has very attractive material and manufacturing properties from a lab-on-chip perspective, such as, chemically stable surfaces, low absorption of small molecules, facile and inexpensive manufacturing process and a biocompatible bonding method. As the OSTE-platform can mirror many of the properties of commercially used polymers, while at the same time having an inexpensive and facile manufacturing method, it has potential to bridge the gap between research and commercial production.

Friction in liquid flows is a critical limiting factor in microfluidics, where friction is the dominant force, but also in marine applications where frictional losses are responsible for a large part of the total energy consumption of sea vessels. Microstructured surfaces can drastically reduce the frictional losses by trapping a layer of air bubbles on the surface that can act as an air bearing for the liquid flow. The problem is that these trapped air bubbles collapse at the liquid pressures encountered in practical applications.

The last part of the thesis is devoted to the development of novel low fluidfriction surfaces with increased robustness but also with active control of the surface friction. The results show that the novel surfaces can resist up to three times higher liquid pressure than previous designs, while keeping the same friction reducing capacity. The novel designs represent the first step towards practical implementation of micro-structured surfaces for friction reduction.

Ort, förlag, år, upplaga, sidor
Stockholm: KTH Royal Institute of Technology, 2011. s. xiii, 87
Serie
Trita-EE, ISSN 1653-5146 ; 2011:058
Nyckelord
microsystem technology, MEMS, microfluidics, polymers, off-stoichiometry thiol-ene, point-of-care, lab-on-chip
Nationell ämneskategori
Elektroteknik och elektronik
Identifikatorer
urn:nbn:se:kth:diva-38605 (URN)978-91-7501-086-1 (ISBN)
Disputation
2011-09-23, F3, Lindstedtsvägen 26, KTH, Stockholm, 10:00 (Engelska)
Opponent
Handledare
Anmärkning

QC 20110907

Tillgänglig från: 2011-09-02 Skapad: 2011-08-30 Senast uppdaterad: 2012-09-03Bibliografiskt granskad

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Carlborg, Carl FredrikHaraldsson, Tommyvan der Wijngaart, Wouter
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Elektroteknik och elektronik

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